1EML

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1996-11-20
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	89.700, 89.700, 130.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.201
Rwork	0.201
R-free	0.28500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ema
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.102 *
Number of reflections	14046
Completeness [%]	97.6	87.8
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	20 *	PROTEIN WAS CRYSTALLIZED BY HANGING DROP METHOD. PROTEIN SOLUTION: 17 MG/ML IN 20 MM TRISCL WELL SOLUTION: 2.0 M AS, 100 MM TRISCL, PH 8.5 (HAMPTON SCREEN I, SOLUTION 4) PROTEIN:WELL 1:1, vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	13-22 (mg/ml)
2	1	reservoir	ammonium sulfate	1.8-2.2 (M)
3	1	reservoir	TrisSO4	100 (mM)