1EMD
CRYSTAL STRUCTURE OF A TERNARY COMPLEX OF ESCHERICHIA COLI MALATE DEHYDROGENASE, CITRATE AND NAD AT 1.9 ANGSTROMS RESOLUTION
Experimental procedure
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.800, 43.050, 83.740 |
| Unit cell angles | 90.00, 130.10, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.651 * |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 1.930 * | |
| Rmerge | 0.032 * | |
| Total number of observations | 47867 * | |
| Number of reflections | 18364 * | |
| Completeness [%] | 76.0 * | |
| Redundancy | 2.6 * | 2.6 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.7 * | referred to J.Mol.Biol. 220,551-553 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.6 (mg/ml) | |
| 10 | 1 | reservoir | 0.05 (%) | ||
| 11 | 1 | reservoir | PEG8000 | 15-18 (%) | |
| 2 | 1 | drop | PEG8000 | 14-16 (%(w/v)) | |
| 3 | 1 | drop | sodium citrate | 10 (mM) | |
| 4 | 1 | drop | EDTA | 1 (mM) | |
| 5 | 1 | drop | DTT | 2 (mM) | |
| 6 | 1 | drop | 0.05 (%) | ||
| 7 | 1 | reservoir | sodium citrate | 10 (mM) | |
| 8 | 1 | reservoir | EDTA | 1 (mM) | |
| 9 | 1 | reservoir | DTT | 2 (mM) |
