1EMA
GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-28 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.770, 62.850, 70.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.175 * |
| Structure solution method | MIR/SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 19.650 * |
| Data reduction software | HKL |
| Data scaling software | HKL |
| Phasing software | CCP4 |
| Refinement software | TNT (5-F) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.090 |
| Number of reflections | 17676 |
| Completeness [%] | 84.0 |
| Redundancy | 6.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.2 | 22-26% PEG 4000, 50 MM HEPES PH 8.0-8.4, 50 MM MGCL2, 10 MM 2-MERCAPTOETHANOL, 5-7 MG PROTEIN, pH 8.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 22-26 (%) | |
| 2 | 1 | reservoir | HEPES | 50 (mM) | |
| 3 | 1 | reservoir | 50 (mM) | ||
| 4 | 1 | reservoir | 2-mercaptoethanol | 10 (mM) |
