1ELW

Crystal structure of the TPR1 domain of HOP in complex with a HSC70 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-02
Detector	ADSC QUANTUM 4
Spacegroup name	P 41
Unit cell lengths	75.470, 75.470, 42.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.870 - 1.600
R-factor	0.18
Rwork	0.180
R-free	0.21500
RMSD bond length	0.008
RMSD bond angle	1.300
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.041	0.305
<I/σ(I)>	32.5
Completeness [%]	95.8	75.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	20 *	used to microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein-peptide complex	20 (mg/ml)
2	1	drop	HEPES	15 (mM)
3	1	reservoir	Tris	100 (mM)
4	1	reservoir	PEG2000 MME	24 (%(w/v))
5	1	reservoir		10 (mM)
6	1	reservoir	xylitol	15 (%(w/v))