1ELS

CATALYTIC METAL ION BINDING IN ENOLASE: THE CRYSTAL STRUCTURE OF ENOLASE-MN2+-PHOSPHONOACETOHYDROXAMATE COMPLEX AT 2.4 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 42 21 2
Unit cell lengths	124.100, 124.100, 66.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.600 *
R-factor	0.165
RMSD bond length	0.013
RMSD bond angle	0.048
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.450 *
Total number of observations	36445 *
Number of reflections	16561 *
Completeness [%]	83.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5 *		Lebioda, L., (1984) J.Mol.Biol., 180, 213. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (%)
2	1	drop		2 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	dithiothreitol	1 (mM)
5	1	drop	citrate	0.05 (M)
6	1	drop	ammonium sulfate	55 (%sat)
7	1	reservoir	ammonium sulphate	55 (%sat)