1ELR
Crystal structure of the TPR2A domain of HOP in complex with the HSP90 peptide MEEVD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-05 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 73.280, 48.270, 38.060 |
| Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
| Resolution | 9.930 - 1.900 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.21900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MLPHARE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 | 0.068 |
| <I/σ(I)> | 22.8 | |
| Completeness [%] | 97.1 | 91.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 20 * | PEG MME 2000, TRIS, Nickel chloride, Xylitol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein-peptide complex | 15 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | DDT | 2 (mM) | |
| 4 | 1 | reservoir | Tris | 100 (mM) | |
| 5 | 1 | reservoir | PEG2000MME | 20 (%(w/v)) | |
| 6 | 1 | reservoir | 5 (mM) | ||
| 7 | 1 | reservoir | xylitol | 10 (%(w/v)) |
