1ELF
NATURE OF THE INACTIVATION OF ELASTASE BY N-PEPTIDYL-O-AROYL HYDROXYLAMINE AS A FUNCTION OF PH
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.270, 58.090, 75.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 * - 1.700 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 3.900 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.360 * |
| High resolution limit [Å] | 1.680 * |
| Rmerge | 0.100 * |
| Number of reflections | 23713 * |
| Completeness [%] | 91.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.0-7.4 (mg/ml) | |
| 2 | 1 | drop | sodium sulfate | 5-11 (mM) | |
| 3 | 1 | drop | sodium acetate | 10 (mM) | |
| 4 | 1 | reservoir | sodium sulphate | 20 (mM) | |
| 5 | 1 | reservoir | acetate | 10 (mM) |
