1EL9

COMPLEX OF MONOMERIC SARCOSINE OXIDASE WITH THE INHIBITOR [METHYLTHIO]ACETATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-07-23
Detector	RIGAKU RAXIS
Spacegroup name	P 1 21 1
Unit cell lengths	72.762, 69.587, 73.765
Unit cell angles	90.00, 94.02, 90.00

Refinement procedure

Resolution	500.000 - 2.000
R-factor	0.174
Rwork	0.174
R-free	0.21600
RMSD bond length	0.009
RMSD bond angle	1.680
Data scaling software	SCALEPACK
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.088	0.250
Number of reflections	43804
<I/σ(I)>	10.3
Completeness [%]	88.4	53.6
Redundancy	3.8	2.55

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	295	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	sodium/potassium phosphate	2.1 (M)