1EL7

COMPLEX OF MONOMERIC SARCOSINE OXIDASE WITH THE INHIBITOR [METHYTELLURO]ACETATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-11
Detector	RIGAKU RAXIS
Spacegroup name	P 1 21 1
Unit cell lengths	72.722, 69.504, 73.465
Unit cell angles	90.00, 94.12, 90.00

Refinement procedure

Resolution	500.000 - 1.900
R-factor	0.175
Rwork	0.175
R-free	0.21300
RMSD bond length	0.008
RMSD bond angle	1.630
Data scaling software	SCALEPACK
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.091	0.248
Number of reflections	52916
<I/σ(I)>	17.7
Completeness [%]	94.2	62.3
Redundancy	2.8	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	295	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	sodium/potassium phosphate	2.1 (M)