1EL6

STRUCTURE OF BACTERIOPHAGE T4 GENE PRODUCT 11, THE INTERFACE BETWEEN THE BASEPLATE AND SHORT TAIL FIBERS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-22
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	63.930, 99.423, 129.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.000
R-factor	0.2081
Rwork	0.208
R-free	0.22890
RMSD bond length	0.005
RMSD bond angle	1.209
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.710	2.010
High resolution limit [Å]	2.000	2.000
Rmerge	0.058	0.224
Total number of observations	229858 *
Number of reflections	54537
<I/σ(I)>	39.1
Completeness [%]	96.3	99.4 *
Redundancy	4.49	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	dioxane, tris chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	40 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	dioxane	35 (%(v/v))