1EL5

COMPLEX OF MONOMERIC SARCOSINE OXIDASE WITH THE INHIBITOR DIMETHYLGLYCINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-11-10
Detector	RIGAKU RAXIS
Spacegroup name	P 1 21 1
Unit cell lengths	72.865, 69.503, 73.498
Unit cell angles	90.00, 94.25, 90.00

Refinement procedure

Resolution	500.000 - 1.800
R-factor	0.165
Rwork	0.165
R-free	0.20800
RMSD bond length	0.013
RMSD bond angle	1.810
Data scaling software	SCALEPACK
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.057	0.333
Number of reflections	60143
<I/σ(I)>	26.3
Completeness [%]	88.3	36.7
Redundancy	4.3	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	295	drop consists of equal amounts of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	sodium/potassium phosphate	2.1 (M)