1EKM

CRYSTAL STRUCTURE AT 2.5 A RESOLUTION OF ZINC-SUBSTITUTED COPPER AMINE OXIDASE OF HANSENULA POLYMORPHA EXPRESSED IN ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1998-09-25
Detector	RIGAKU RAXIS
Spacegroup name	C 2 2 21
Unit cell lengths	139.227, 153.515, 223.407
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	500.000 - 2.500
R-factor	0.183
Rwork	0.183
R-free	0.20800
RMSD bond length	0.009
RMSD bond angle	1.690
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.087	0.185
Total number of observations	226364 *
Number of reflections	64796
<I/σ(I)>	11.8
Completeness [%]	77.4 *	40.7
Redundancy	3.5	2.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG 8000, potassium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 295.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	PEG8000	9-14 (%)
3	1	drop	potassium phosphate	100-300 (mM)