1EK3

KAPPA-4 IMMUNOGLOBULIN VL, REC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-08-08
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	36.580, 66.910, 91.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.990 - 1.900
R-factor	0.187
Rwork	0.187
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Len_Q38E (PDB 3LVE)
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.032	0.091
Number of reflections	17825
<I/σ(I)>	53.7	17.8
Completeness [%]	96.9	82.2
Redundancy	11.5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	10% PEG8000, 0.05M Calcium Acetate, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K