1EJY

MOUSE IMPORTIN ALPHA-NUCLEOPLASMIN NLS PEPTIDE COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	1999-09-09
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	78.901, 89.828, 99.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.900
R-factor	0.223
Rwork	0.223
R-free	0.26800
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.000
High resolution limit [Å]	2.900	2.900
Rmerge	0.239	0.962
Total number of observations	121070 *
Number of reflections	16370	1598 *
<I/σ(I)>	7.2
Completeness [%]	98.9	100
Redundancy	7.4	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5	293	sodium citrate, Hepes, DTT, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium citrate	0.55 (M)
2	1	1	HEPES	100 (mM)
3	1	1	dithiothreitol	10 (mM)