1EJR
CRYSTAL STRUCTURE OF THE D221A VARIANT OF KLEBSIELLA AEROGENES UREASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 298 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IV |
Spacegroup name | I 21 3 |
Unit cell lengths | 170.600, 170.600, 170.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.161 * |
Rwork | 0.161 |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.090 * | 0.580 * |
Number of reflections | 55503 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.8 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 25 * | Jabri, E., (1992) J.Mol.Biol., 227, 934. * |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 25 * | Jabri, E., (1992) J.Mol.Biol., 227, 934. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | urease | 5 (mg/ml) | |
2 | 1 | drop | Tris | 10 (mM) | |
3 | 1 | drop | EDTA | 0.5 (mM) | |
4 | 1 | drop | beta-mercaptoethanol | 0.5 (mM) | |
5 | 1 | drop | 0.75-0.85 (M) | ||
6 | 1 | drop | HEPES | 50 (mM) | |
7 | 1 | reservoir | 1.5-1.7 (M) | ||
8 | 1 | reservoir | HEPES | 100 (mM) |