1EJE

CRYSTAL STRUCTURE OF AN FMN-BINDING PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-12-08
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 21 2
Unit cell lengths	94.650, 94.650, 45.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.930 - 2.200
R-factor	0.225
Rwork	0.225
R-free	0.27900
RMSD bond length	0.016
RMSD bond angle	24.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.310
High resolution limit [Å]	2.200	2.200
Rmerge	0.047	0.261
Number of reflections	85613
<I/σ(I)>	29
Completeness [%]	100.0	100
Redundancy	8.5	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6	295	LiSO4, MES, NiCl2, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 22K