1EJC

Crystal structure of unliganded mura (type2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1999-05-05
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	67.385, 73.668, 77.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
Rwork	0.167
R-free	0.19200
RMSD bond length	0.008
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.046	0.225
Total number of observations	251049 *
Number of reflections	36451
<I/σ(I)>	28.4
Completeness [%]	99.6	99.2
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	19 *	10 % PEG 20000, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	30-50 (mg/ml)
2	1	drop	PEG20000	10 (%)
3	1	drop	MES	0.1 (M)
4	1	reservoir	MES	0.5 (M)