1EJ7
CRYSTAL STRUCTURE OF UNACTIVATED TOBACCO RUBISCO WITH BOUND PHOSPHATE IONS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | MACSCIENCE |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 1997-05-25 |
Detector | MACSCIENCE |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 149.180, 149.180, 138.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.450 |
Rwork | 0.149 |
R-free | 0.19700 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.084 | 0.339 |
Number of reflections | 27448 | |
<I/σ(I)> | 12 | |
Completeness [%] | 95.1 | 92.2 |
Redundancy | 3.45 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | liquid-liquid diffusion * | 5.2 | 25 * | 10microL of (tobacco rubisco 20-50mg/ml in (tris-Cl 50mm pH 7.4 at 4degC, MgCl2 20mM, NaHCO3 20mM, EDTA 0.1mM, beta-mercaptoethanol 50mM)) and 35 microL of (K2HPO4 200mM, NH4K2PO4 300mM, NaN3, plus HCl to pH 5.2 at 24degC) is sealed, in contact, in a 7mm glass capillary, LIQUID DIFFUSION, temperature 295K |
1 | liquid-liquid diffusion * | 5.2 | 25 * | 10microL of (tobacco rubisco 20-50mg/ml in (tris-Cl 50mm pH 7.4 at 4degC, MgCl2 20mM, NaHCO3 20mM, EDTA 0.1mM, beta-mercaptoethanol 50mM)) and 35 microL of (K2HPO4 200mM, NH4K2PO4 300mM, NaN3, plus HCl to pH 5.2 at 24degC) is sealed, in contact, in a 7mm glass capillary, LIQUID DIFFUSION, temperature 295K |
1 | liquid-liquid diffusion * | 5.2 | 25 * | 10microL of (tobacco rubisco 20-50mg/ml in (tris-Cl 50mm pH 7.4 at 4degC, MgCl2 20mM, NaHCO3 20mM, EDTA 0.1mM, beta-mercaptoethanol 50mM)) and 35 microL of (K2HPO4 200mM, NH4K2PO4 300mM, NaN3, plus HCl to pH 5.2 at 24degC) is sealed, in contact, in a 7mm glass capillary, LIQUID DIFFUSION, temperature 295K |
1 | liquid-liquid diffusion * | 5.2 | 25 * | 10microL of (tobacco rubisco 20-50mg/ml in (tris-Cl 50mm pH 7.4 at 4degC, MgCl2 20mM, NaHCO3 20mM, EDTA 0.1mM, beta-mercaptoethanol 50mM)) and 35 microL of (K2HPO4 200mM, NH4K2PO4 300mM, NaN3, plus HCl to pH 5.2 at 24degC) is sealed, in contact, in a 7mm glass capillary, LIQUID DIFFUSION, temperature 295K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | 0.2 (M) | ||
2 | 1 | 1 | 0.3 (M) | ||
4 | 1 | 1 | phosphate | 389 (mM) |