1EIQ

2,3-DIHYDROXYBIPHENYL-1,2-DIOXYGENASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-10-28
Detector	RIGAKU RAXIS IIC
Spacegroup name	I 4 2 2
Unit cell lengths	121.890, 121.890, 108.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.000 - 2.000
R-factor	0.188
Rwork	0.184
R-free	0.23400 *
RMSD bond length	0.014 *
RMSD bond angle	2.100 *
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.250
High resolution limit [Å]	2.000 *	1.980
Rmerge	0.082	0.201
Number of reflections	24620 *
<I/σ(I)>	11.5
Completeness [%]	88.1 *	83.5
Redundancy	2.6	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5	12 *	285K, Tris/HCl, Ammonium sulfate, hexylene glycol, pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	Tris-HCl	100 (mM)
3	1	1	ammonium sulfate	27.7 (%sat)
4	1	1	hexylene glycol	18 (%(v/v))