1EIL
2,3-DIHYDROXYBIPHENYL-1,2-DIOXYGENASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1999-12-07 |
| Detector | WEISSENBERG |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 121.750, 121.750, 108.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 2.000 |
| R-factor | 0.209 |
| Rwork | 0.206 |
| R-free | 0.26500 * |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 2.400 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 * | 0.255 |
| Number of reflections | 27837 * | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 99.8 | 99.9 * |
| Redundancy | 3.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.5 | 12 * | 285 K, Tris/HCl Ammonium sulfate hexylene glycol, pH 7.5, temperature 285.0K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 100 (mM) | |
| 3 | 1 | 1 | ammonium sulfate | 27.7 (%sat) | |
| 4 | 1 | 1 | hexylene glycol | 18 (%(v/v)) |
