1EIL

2,3-DIHYDROXYBIPHENYL-1,2-DIOXYGENASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	DIFFRACTOMETER
Collection date	1999-12-07
Detector	WEISSENBERG
Spacegroup name	I 4 2 2
Unit cell lengths	121.750, 121.750, 108.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.000
R-factor	0.209
Rwork	0.206
R-free	0.26500 *
RMSD bond length	0.016 *
RMSD bond angle	2.400 *
Data reduction software	DENZO
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.060 *	0.255
Number of reflections	27837 *
<I/σ(I)>	10.5
Completeness [%]	99.8	99.9 *
Redundancy	3.6	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7.5	12 *	285 K, Tris/HCl Ammonium sulfate hexylene glycol, pH 7.5, temperature 285.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	Tris-HCl	100 (mM)
3	1	1	ammonium sulfate	27.7 (%sat)
4	1	1	hexylene glycol	18 (%(v/v))