1EID
CRYSTAL STRUCTURE OF F120G MUTANT OF BOVINE PANCREATIC RIBONUCLEASE A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL40B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL40B2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-11-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.733, 63.733, 63.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 * - 1.400 |
| R-factor | 0.21 * |
| Rwork | 0.210 |
| R-free | 0.26500 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 * | 1.240 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.043 | 0.307 |
| Total number of observations | 404905 * | |
| Number of reflections | 41727 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 88.8 | 87.3 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 25 * | ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 24 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 100 (mM) | pH6.0 |
| 3 | 1 | reservoir | ammonium sulfate | 30 (%(v/v)saturated) | |
| 4 | 1 | reservoir | 50 (%(v/v)saturated) |
