1EIC

CRYSTAL STRUCTURE OF F120A MUTANT OF BOVINE PANCREATIC RIBONUCLEASE A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	1999-06-03
Detector	ADSC QUANTUM 4r
Spacegroup name	P 32 2 1
Unit cell lengths	63.661, 63.661, 63.492
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 1.400
R-factor	0.201 *
Rwork	0.201
R-free	0.25400
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.800	1.008
High resolution limit [Å]	1.000	1.000
Rmerge	0.070
Total number of observations	1446353 *
Number of reflections	80089
<I/σ(I)>	36.94
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	25 *	ammonium sulfate, sodium chloride, sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	reservoir	sodium acetate	100 (mM)	pH6.0
3	1	reservoir	ammonium sulfate	34 (%(v/v)saturated)
4	1	reservoir		50 (%(v/v)saturated)