1EI7

TMV COAT PROTEIN REFINED FROM THE 4-LAYER AGGREGATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	113
Detector technology	AREA DETECTOR
Collection date	1996-04-01
Detector	SIEMENS
Spacegroup name	P 21 21 2
Unit cell lengths	220.845, 171.701, 226.175
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 2.450
R-factor	0.195 *
Rwork	0.195
R-free	0.22500
RMSD bond length	0.010
RMSD bond angle	1.970
Data reduction software	X-GEN
Data scaling software	XDS
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.900	29.900
High resolution limit [Å]	2.400	2.450
Rmerge	0.094	0.094
Total number of observations	429646 *
Number of reflections	218046
<I/σ(I)>	6
Completeness [%]	65.7 *	65
Redundancy	2	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	8	300	0.3 M Ammonium Sulfate, 0.1 M Tris/HCl pH 8.0, 14 mg/ml protein concentration, MICRODIALYSIS, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	14 (mg/ml)
2	1	1	ammonium sulfate	0.2 (M)
3	1	1	Tris-HCl	0.1 (M)
4	1	2	ammonium sulfate	0.3 (M)
5	1	2	Tris-HCl	0.1 (M)