1EI7
TMV COAT PROTEIN REFINED FROM THE 4-LAYER AGGREGATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 113 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-04-01 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 220.845, 171.701, 226.175 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.900 - 2.450 |
| R-factor | 0.195 * |
| Rwork | 0.195 |
| R-free | 0.22500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.970 |
| Data reduction software | X-GEN |
| Data scaling software | XDS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 29.900 |
| High resolution limit [Å] | 2.400 | 2.450 |
| Rmerge | 0.094 | 0.094 |
| Total number of observations | 429646 * | |
| Number of reflections | 218046 | |
| <I/σ(I)> | 6 | |
| Completeness [%] | 65.7 * | 65 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 8 | 300 | 0.3 M Ammonium Sulfate, 0.1 M Tris/HCl pH 8.0, 14 mg/ml protein concentration, MICRODIALYSIS, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 14 (mg/ml) | |
| 2 | 1 | 1 | ammonium sulfate | 0.2 (M) | |
| 3 | 1 | 1 | Tris-HCl | 0.1 (M) | |
| 4 | 1 | 2 | ammonium sulfate | 0.3 (M) | |
| 5 | 1 | 2 | Tris-HCl | 0.1 (M) |
