1EI3

CRYSTAL STRUCTURE OF NATIVE CHICKEN FIBRINOGEN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	298
Detector technology	CCD
Collection date	1999-10-29
Detector	BRANDEIS
Spacegroup name	P 1 21 1
Unit cell lengths	114.726, 101.849, 210.232
Unit cell angles	90.00, 106.71, 90.00

Refinement procedure

Resolution	? - 5.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	5.000	5.500 *
Rmerge	0.090
Total number of observations	45940 *
Number of reflections	16412 *
<I/σ(I)>	7.5
Completeness [%]	80.0	65.4 *
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7 *	298	PEG 3350, imidazole, calcium chloride, pH 6.75, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop		0.15 (M)
3	1	drop	imidazole	0.05 (M)
4	1	reservoir	PEG3350	3-4 (%(v/v))
5	1	reservoir	imidazole	0.05 (M)
6	1	reservoir		2 (mM)
7	1	reservoir	sodium azide	1 (mM)