1EHY
X-ray structure of the epoxide hydrolase from agrobacterium radiobacter ad1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1997-08-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 146.624, 100.201, 96.879 |
Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.100 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.22700 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 23.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | X-PLOR (3.843) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.062 | 0.360 |
Total number of observations | 222880 * | |
Number of reflections | 73445 | |
<I/σ(I)> | 14.7 | 2.57 |
Completeness [%] | 91.5 | 82 |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 1.8 M KH2PO4/K2HPO4, pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5.5 (mg/ml) | |
2 | 1 | reservoir | potassium phosphate | 1.6-1.8 (M) |