1EHW
HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 112.960, 112.960, 112.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.22900 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.105 * |
| Total number of observations | 115939 * |
| Number of reflections | 19054 |
| Completeness [%] | 94.3 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | lithium sulfate, sodium chloride, HEPES, EDTA, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 150 (mM) | ||
| 2 | 1 | drop | EDTA | 5 (mM) | |
| 3 | 1 | drop | HEPES | 100 (mM) | |
| 4 | 1 | drop | 0.7 (M) | ||
| 5 | 1 | reservoir | 150 (mM) | ||
| 6 | 1 | reservoir | EDTA | 5 (mM) | |
| 7 | 1 | reservoir | HEPES | 100 (mM) | |
| 8 | 1 | reservoir | 1.4 (M) | ||
| 9 | 1 | drop | protein | 4 (mg/ml) |
