1EGZ

CELLULASE CEL5 FROM ERWINIA CHRYSANTHEMI, A FAMILY GH 5-2 ENZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	287
Detector technology	IMAGE PLATE
Collection date	1995-12-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	168.800, 47.200, 120.100
Unit cell angles	90.00, 105.00, 90.00

Refinement procedure

Resolution	29.000 - 2.300
R-factor	0.187 *
Rwork	0.179
R-free	0.22600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1a3h
RMSD bond length	0.007
RMSD bond angle	22.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.086 *	0.373 *
Number of reflections	38365	1749 *
<I/σ(I)>	5	1.5
Completeness [%]	98.0	97
Redundancy	2.8	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8	4 *	VAPOR DIFFUSION HANGING DROP; PEG 4000 28%, TRIS PH 8.0 20 MM, MGSO4 20 MM, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)	pH8.0
3	1	reservoir	PEG4000	28 (%(w/v))
4	1	reservoir	Tris-HCl	20 (mM)
5	1	reservoir		20 (mM)