1EGZ
CELLULASE CEL5 FROM ERWINIA CHRYSANTHEMI, A FAMILY GH 5-2 ENZYME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1995-12-15 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 168.800, 47.200, 120.100 |
Unit cell angles | 90.00, 105.00, 90.00 |
Refinement procedure
Resolution | 29.000 - 2.300 |
R-factor | 0.187 * |
Rwork | 0.179 |
R-free | 0.22600 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a3h |
RMSD bond length | 0.007 |
RMSD bond angle | 22.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.086 * | 0.373 * |
Number of reflections | 38365 | 1749 * |
<I/σ(I)> | 5 | 1.5 |
Completeness [%] | 98.0 | 97 |
Redundancy | 2.8 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 | 4 * | VAPOR DIFFUSION HANGING DROP; PEG 4000 28%, TRIS PH 8.0 20 MM, MGSO4 20 MM, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | pH8.0 |
3 | 1 | reservoir | PEG4000 | 28 (%(w/v)) | |
4 | 1 | reservoir | Tris-HCl | 20 (mM) | |
5 | 1 | reservoir | 20 (mM) |