1EGW

CRYSTAL STRUCTURE OF MEF2A CORE BOUND TO DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	1999-05-18
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	41.371, 60.696, 63.987
Unit cell angles	115.18, 89.99, 90.00

Refinement procedure

Resolution	20.000 - 1.500
R-factor	0.206 *
Rwork	0.206
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT and heavy atom phases from iodine derivative dataset
RMSD bond length	0.010
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.550
High resolution limit [Å]	1.500	1.500
Rmerge	0.075	0.104
Number of reflections	87264
<I/σ(I)>	12.8
Completeness [%]	96.0	92
Redundancy	2.6	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		22 *	PEG 6000, NaCl, Ba(NO3)2, sodium Acetate, Bis-tris buffer, DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	NaCl
2	1	1	Ba(NO3)2
3	1	1	sodium Acetate
4	1	1	bis-tris buffer
5	1	1	DTT
6	1	1	PEG 6000
7	1	2	PEG 6000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	15-18 (%)
2	1	reservoir		50 (mM)
3	1	reservoir		5 (mM)
4	1	reservoir	Bis-Tris	50 (mM)
5	1	reservoir		25 (mM)
6	1	reservoir	dithiothreitol	2 (mM)