1EGJ

DOMAIN 4 OF THE BETA COMMON CHAIN IN COMPLEX WITH AN ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-01
Detector	OTHER
Spacegroup name	P 41 21 2
Unit cell lengths	77.610, 77.610, 296.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.228 *
Rwork	0.228
R-free	0.28800
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.098	0.620
Total number of observations	58732 *
Number of reflections	21211
<I/σ(I)>	11.4
Completeness [%]	88.1	87.9
Redundancy	2.8	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	12% peg 4000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 22K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.9-4 (mg/ml)
2	1	drop	citrate	0.043 (M)
3	1	drop	PEG4000	5.1 (%(w/v))
4	1	reservoir	citrate	0.100 (M)
5	1	reservoir	PEG4000	12 (%(w/v))