1EGI

STRUCTURE OF A C-TYPE CARBOHYDRATE-RECOGNITION DOMAIN (CRD-4) FROM THE MACROPHAGE MANNOSE RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-01-08
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	130.056, 38.689, 55.025
Unit cell angles	90.00, 98.57, 90.00

Refinement procedure

Resolution	500.000 - 2.300
R-factor	0.212 *
Rwork	0.212
R-free	0.26200
RMSD bond length	0.006
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.064	0.354
Total number of observations	103297 *
Number of reflections	12246
Completeness [%]	99.3	100
Redundancy	8.4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	21 *	8-13% polyethylene glycol, 100 mM Tris pH 8.0, 100 mM NaCl, 10 mM CaCl2, 100 mM a-Me-Man. The crystals were then transferred to a solution containing 200 mM methyl 3-O-(a-D-mannopyranosyl)-a-D-mannopyranoside, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop		3-5 (mM)
3	1	reservoir	PEG10000	8-13 (%)
4	1	reservoir	Tris	100 (mM)
5	1	reservoir		100 (mM)
6	1	reservoir		10 (mM)