1EG5
NIFS-LIKE PROTEIN
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-12-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.25, 0.977250, 0.976430 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 114.110, 114.110, 156.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.920 - 2.000 |
| R-factor | 0.211 |
| Rwork | 0.211 |
| R-free | 0.23200 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 500.000 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.081 * | |
| Total number of observations | 233681 * | |
| Number of reflections | 80563 * | |
| Completeness [%] | 95.7 * | 97.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 * | 291 | ammonium sulphate, hepes, dtt, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 18K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | 10 (mM) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.9 (M) | |
| 4 | 1 | reservoir | NaOH/HEPES | 100 (mM) | pH7.5 |
