1EG5

NIFS-LIKE PROTEIN

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	1998-12-01
Detector	MARRESEARCH
Wavelength(s)	1.25, 0.977250, 0.976430
Spacegroup name	P 31 2 1
Unit cell lengths	114.110, 114.110, 156.960
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.920 - 2.000
R-factor	0.211
Rwork	0.211
R-free	0.23200
RMSD bond length	0.012
RMSD bond angle	1.400
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	500.000	2.130
High resolution limit [Å]	2.000	2.000
Rmerge	0.081 *
Total number of observations	233681 *
Number of reflections	80563 *
Completeness [%]	95.7 *	97.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5 *	291	ammonium sulphate, hepes, dtt, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 18K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	drop	dithiothreitol	10 (mM)
3	1	reservoir	ammonium sulfate	0.9 (M)
4	1	reservoir	NaOH/HEPES	100 (mM)	pH7.5