1EFZ

MUTAGENESIS AND CRYSTALLOGRAPHIC STUDIES OF ZYMOMONAS MOBILIS TRNA-GUANINE TRANSGLYCOSYLASE TO ELUCIDATE THE ROLE OF SERINE 103 FOR ENZYMATIC ACTIVITY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-05-27
Detector	RIGAKU RAXIS IV
Spacegroup name	C 1 2 1
Unit cell lengths	90.600, 65.040, 70.740
Unit cell angles	90.00, 96.25, 90.00

Refinement procedure

Resolution	71.000 - 2.000
Rwork	0.161
R-free	0.19900
RMSD bond length	0.006
RMSD bond angle	1.345
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall
Low resolution limit [Å]	71.000
High resolution limit [Å]	2.000
Rmerge	0.057
Number of reflections	27018
Completeness [%]	97.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	25 *	PEG 8000, tris, dtt, dmso, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris-HCl	100 (mM)
2	1	reservoir	PEG8000	13 (%)
3	1	reservoir	dithiothreitol	1 (mM)