1EFQ
Q38D mutant of LEN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-02-19 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 63 |
| Unit cell lengths | 65.800, 65.800, 48.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.600 |
| R-factor | 0.23 * |
| Rwork | 0.230 |
| R-free | 0.28850 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.570 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.074 | 0.204 |
| Number of reflections | 15317 | |
| <I/σ(I)> | 16.5 | |
| Completeness [%] | 97.3 | 99.7 |
| Redundancy | 16 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 25% PEG monomethylether 550, 0.01 M zinc sulfate, 0.1 M MES pH 6.5, 0.001M Uranyl Acetate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG550 MME | 25 (%) | |
| 2 | 1 | reservoir | zinc sulfate | 0.01 (M) | |
| 3 | 1 | reservoir | MES | 0.1 (M) | pH6.5 |
| 4 | 1 | reservoir | uranyl acetate | 1 (mM) | |
| 5 | 1 | drop | protein | 10 (mg/ml) |
