1EFA
CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-01-11 |
Detector | BRANDEIS - B4 |
Spacegroup name | H 3 2 |
Unit cell lengths | 251.438, 251.438, 204.791 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.600 |
R-factor | 0.247 |
Rwork | 0.247 |
R-free | 0.26400 |
RMSD bond length | 0.007 |
RMSD bond angle | 25.300 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 17.000 | 2.680 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.066 | 0.402 |
Total number of observations | 392400 * | |
Number of reflections | 74896 | |
<I/σ(I)> | 13.5 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 7.5 | 298 | ammonium sulfate, PEG 400, HEPES, Glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | HEPES | ||
2 | 1 | 1 | ammonium sulfate | ||
3 | 1 | 1 | PEG 400 | ||
4 | 1 | 1 | Glycerol | ||
5 | 1 | 2 | ammonium sulfate | ||
6 | 1 | 2 | PEG 400 | ||
7 | 1 | 2 | Glycerol |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | Lac dimer | 14 (mg/ml) | ||
2 | 1 | DNA | 1.5-fold molar excess | ||
3 | 1 | ONPF | 10-fold excess | ||
4 | 1 | 2 | PEG400 | 10 (%) | |
5 | 1 | sodium sulphate | 2.0 (M) | ||
6 | 1 | HEPES | 0.1 (M) | ||
7 | 1 | glycerol | 14 (%) |