1EF1

CRYSTAL STRUCTURE OF THE MOESIN FERM DOMAIN/TAIL DOMAIN COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1998-03-15
Detector	ADSC QUANTUM 4
Spacegroup name	P 2 2 21
Unit cell lengths	54.200, 153.300, 112.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.227 *
Rwork	0.227
R-free	0.28700
RMSD bond length	0.016
RMSD bond angle	1.700
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MADSYS
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.090 *	0.445 *
Number of reflections	71584
Completeness [%]	95.0	92
Redundancy	3.6	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	PEG3350, lithium sulfate, HEPES, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	PEG3350, lithium sulfate, HEPES, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	HEPES	10 (mM)
3	1	drop		20 (mM)
4	1	drop	EDTA	1 (mM)
5	1	drop	dithiothreitol	5 (mM)
6	1	reservoir	HEPES	100 (mM)
7	1	reservoir		100-150 (mM)
8	1	reservoir	PEG3350	18-20 (%)