1EDU

CRYSTAL STRUCTURE OF THE ENTH DOMAIN OF RAT EPSIN 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	105
Detector technology	CCD
Collection date	1999-06-15
Detector	ADSC QUANTUM 4
Spacegroup name	P 31 2 1
Unit cell lengths	49.807, 49.807, 99.967
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	500.000 - 1.800
Rwork	0.206
R-free	0.22400
RMSD bond length	0.005
RMSD bond angle	18.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.055	0.202 *
Number of reflections	24057
<I/σ(I)>	25.26
Completeness [%]	93.6	68.3
Redundancy	5.44 *	2.03 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 4000, Na-HEPES, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 4000, Na-HEPES, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 4000, Na-HEPES, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 4000, Na-HEPES, ethylene glycol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 20K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7.5 (mg/ml)
2	1	reservoir	sodium-HEPES	100 (mM)
3	1	reservoir	PEG1000	10 (%)
4	1	reservoir	ethylene glycol	8 (%)