1EDT
CRYSTAL STRUCTURE OF ENDO-BETA-N-ACETYLGLUCOSAMINIDASE H AT 1.9 ANGSTROMS RESOLUTION: ACTIVE SITE GEOMETRY AND SUBSTRATE RECOGNITION
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.070, 85.070, 89.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.590 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.090 * |
| Total number of observations | 89595 * |
| Number of reflections | 29155 |
| Completeness [%] | 83.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 18 (%(w/v)) | |
| 2 | 1 | reservoir | zinc acetate | 200 (mM) | |
| 3 | 1 | reservoir | cacodylate | 100 (mM) |
