1EDO
THE X-RAY STRUCTURE OF BETA-KETO ACYL CARRIER PROTEIN REDUCTASE FROM BRASSICA NAPUS COMPLEXED WITH NADP+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 129.280, 129.280, 92.640 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.300 |
| R-factor | 0.19 * |
| Rwork | 0.190 |
| R-free | 0.23500 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 2.340 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.071 | 0.450 |
| Total number of observations | 102604 * | |
| Number of reflections | 21256 * | |
| Completeness [%] | 95.0 | 91.5 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 290 | Fisher, M., (2000) Acta Crystallogr., Sect.D, 56, 86. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 80 (%) | |
| 2 | 1 | drop | sodium phosphate | 50 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | NADP+ | 5 (mM) | |
| 5 | 1 | reservoir | PEG1500 | 8-12 (%(w/v)) | |
| 6 | 1 | reservoir | sodium citrate | 100 (mM) |
