1ECW

CRYSTAL STRUCTURE OF SIMIAN IMMUNODEFICIENCY VIRUS MATRIX ANTIGEN (SIV MA) AT 293K.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-01-01
Detector	MARRESEARCH
Spacegroup name	H 3
Unit cell lengths	114.900, 114.900, 32.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.200
Rwork	0.164
RMSD bond length	0.009
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.200
Rmerge	0.072
Number of reflections	7686
Completeness [%]	98.2
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	PEG 8000, isopropanol, Tris-HCL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
1	VAPOR DIFFUSION, SITTING DROP	8	293	PEG 8000, isopropanol, Tris-HCL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	PEG3600	9 (%)
3	1	drop	isopropanol	7.5 (%)
4	1	drop	Tris-HCl	25 (mM)
5	1	reservoir	PEG8000	25 (%(w/v))
6	1	reservoir	isopropanol	15 (%(v/v))
7	1	reservoir	Tris-HCl	50 (mM)