1ECL
AMINO TERMINAL 67KDA DOMAIN OF ESCHERICHIA COLI DNA TOPOISOMERASE I (RESIDUES 2-590 OF MATURE PROTEIN) CLONING ARTIFACT ADDS TWO RESIDUES TO THE AMINO-TERMINUS WHICH WERE NOT OBSERVED IN THE EXPERIMENTAL ELECTRON DENSITY (GLY-2, SER-1).
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS |
| Detector technology | CCD |
| Collection date | 1994-10-23 |
| Detector | PRINCETON 2K |
| Wavelength(s) | 0.9144 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.190, 77.970, 138.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.900 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.868 |
| Data reduction software | DENZO |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.127 * |
| High resolution limit [Å] | 1.604 * |
| Rmerge | 0.059 |
| Number of reflections | 56798 |
| Completeness [%] | 80.0 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 23 * | Lima, C.D., (1993) J.Mol.Biol., 232, 1213. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2-10 (mg/ml) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 20 (mM) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) |
