1ECG
DON INACTIVATED ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-23 |
| Detector | RIGAKU |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.900, 157.500, 106.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.147 |
| Rwork | 0.147 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ecf |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.980 * |
| Data reduction software | HKL ((DENZO)) |
| Data scaling software | HKL |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.054 | 0.170 |
| Total number of observations | 192620 * | |
| Number of reflections | 40313 | |
| <I/σ(I)> | 24.2 | 5.6 |
| Completeness [%] | 91.8 | 61.2 |
| Redundancy | 4.8 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.3 * | 20 * | drop consists of equal volume of enzyme and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 18.5 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 13 (%) | |
| 3 | 1 | reservoir | 2-propanol | 5 (%) | |
| 4 | 1 | reservoir | PIPES | 100 (mM) | |
| 5 | 1 | reservoir | 4 (mM) | ||
| 6 | 1 | reservoir | 4 (mM) |
