1EC5

CRYSTAL STRUCTURE OF FOUR-HELIX BUNDLE MODEL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-02-09
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	36.070, 89.160, 79.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.500
Rwork	0.237
R-free	0.30400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	THEORETICAL MODEL
RMSD bond length	0.009
RMSD bond angle	0.036
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.400	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.107	0.480
Total number of observations	23396 *
Number of reflections	4717 *
<I/σ(I)>	11.7	2.7
Completeness [%]	95.5 *	96.7
Redundancy	5.2	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	277	CRYSTALS WERE GROWN FROM AN AMMONIUM SULFATE 2M SOLUTION, pH 4.6, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium acetate	0.1 (M)
2	1	reservoir	ammonium sulfate	2.0 (M)