1EBT

HEMOGLOBIN I FROM THE CLAM LUCINA PECTINATA BOUND WITH CYANIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	300
Detector technology	IMAGE PLATE
Collection date	1995-12
Detector	RIGAKU RAXIS
Spacegroup name	P 1 21 1
Unit cell lengths	50.670, 38.760, 42.530
Unit cell angles	90.00, 106.93, 90.00

Refinement procedure

Resolution	24.000 - 1.900
R-factor	0.184 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1flp
RMSD bond length	0.015
RMSD bond angle	2.400 *
Data reduction software	MOSFLM
Phasing software	AMoRE
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000
High resolution limit [Å]	1.900	1.900 *
Rmerge	0.054 *
Number of reflections	12085
Completeness [%]	93.3 *
Redundancy	4.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.75 *		pH 7.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	myoglobin	6 (%)
2	1	1	ammonium sulfate	100 (%)