1EBO

CRYSTAL STRUCTURE OF THE EBOLA VIRUS MEMBRANE-FUSION SUBUNIT, GP2, FROM THE ENVELOPE GLYCOPROTEIN ECTODOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1998-08-15
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	171.702, 32.691, 168.863
Unit cell angles	90.00, 119.23, 90.00

Refinement procedure

Resolution	20.000 - 3.000
Rwork	0.239
R-free	0.25600
Structure solution method	SIR
RMSD bond length	0.016
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.090
High resolution limit [Å]	3.000	3.000
Rmerge	0.083 *	0.290 *
Total number of observations	87380 *
Number of reflections	17123
Completeness [%]	99.3	99.2
Redundancy	5.1	3.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.3 *		0.02ml of protein solution was mixed with 0.01ml of reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	Tris-HCl	100 (mM)
4	1	reservoir	PEG4000	30-34 (%)
5	1	reservoir	ammonium sulfate	220 (mM)
6	1	reservoir	dioxane	1.0 (%)