1EAY
CHEY-BINDING (P2) DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM ESCHERICHIA COLI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.500, 64.200, 158.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| Rwork | 0.217 |
| Structure solution method | MIR AND MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3CHY AND 1FWP |
| RMSD bond length | 0.022 |
| RMSD bond angle | 19.140 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PROTEIN |
| Refinement software | TNT (5-F) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.055 |
| Total number of observations | 146840 * |
| Number of reflections | 30701 |
| Completeness [%] | 78.0 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | PROTEIN WAS CRYSTALLIZED FROM .63 M NAH2PO4/1.17 M K2HPO4, 10 MM NH4CL, PH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | CheY | 3.8 (mg/ml) | |
| 2 | 1 | drop | P2 | 2.3 (mg/ml) | |
| 3 | 1 | drop | sodium phosphate | 0.32 (M) | |
| 4 | 1 | drop | potassium phosphate | 0.59 (M) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | reservoir | sodium phosphate | 0.63 (M) | |
| 7 | 1 | reservoir | potassium phosphate | 1.17 (M) | |
| 8 | 1 | reservoir | 10 (mM) |
