1EAN

THE RUNX1 Runt domain at 1.70A resolution: A structural switch and specifically bound chloride ions modulate DNA binding

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	1999-06-06
Detector	ADSC CCD
Spacegroup name	H 3 2
Unit cell lengths	110.696, 110.696, 117.293
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.700 * - 1.700
Rwork	0.204
R-free	0.22300 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB CODE 1EAQ
RMSD bond length	0.014 *
RMSD bond angle	2.140 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	REFMAC (5.0.36)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.040	0.580
Total number of observations	357303 *
Number of reflections	28866
<I/σ(I)>	11.3	1.5
Completeness [%]	99.7	99
Redundancy	5.7	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *	293 *	30% MPEG 350, 5-10% PEG 3350, 70 MM NA CACODYLATE, PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16 (mg/ml)
2	1	drop		1.1 (M)
3	1	reservoir	MPEG350	30 (%(v/v))
4	1	reservoir	PEG3350	5-10 (%(w/v))
5	1	reservoir	HEPES	70 (mM)	pH7.0