1EA6
N-terminal 40kDa fragment of NhPMS2 complexed with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-03-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.043, 74.983, 135.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.690 - 2.700 |
| R-factor | 0.23 |
| Rwork | 0.230 |
| R-free | 0.26200 |
| Structure solution method | OTHER |
| RMSD bond length | 0.010 * |
| RMSD bond angle | 1.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.064 | 0.304 |
| Number of reflections | 19940 | |
| <I/σ(I)> | 12.5 | 2.1 |
| Completeness [%] | 92.3 | 91.7 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 2-2.4 M NA/K PHOSPHATE (PH 6.2) 0.2 M LICL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium potassium phosphate | 2-2.4 (M) | pH6.2 |
| 2 | 1 | reservoir | 0.2 (M) | ||
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | Tris | 20 (mM) | pH8.5 |
| 5 | 1 | drop | 200 (mM) | ||
| 6 | 1 | drop | EDTA | 0.1 (mM) | |
| 7 | 1 | drop | dithiothreitol | 5 (mM) | |
| 8 | 1 | drop | glycerol | 5 (%) |
