1EA0
Alpha subunit of A. brasilense glutamate synthase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 233.612, 233.612, 305.089 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.25618 |
| Rwork | 0.256 |
| R-free | 0.28712 |
| Structure solution method | MAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 0.027 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.092 | 0.456 |
| Total number of observations | 758585 * | |
| Number of reflections | 184182 | |
| <I/σ(I)> | 6.4 | 1.2 |
| Completeness [%] | 98.6 | 94.1 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | 20 * | pH 5.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 10 | 1 | reservoir | PEG3350 | 15 (%(w/v)) | |
| 11 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 2 | 1 | drop | EDTA | 1 (mM) | |
| 3 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 4 | 1 | drop | MetS | 2 (mM) | |
| 5 | 1 | drop | 2-oxoglutarate | 2 (mM) | |
| 6 | 1 | drop | AADP | 2 (mM) | |
| 7 | 1 | drop | dithiothreitol | 5 (mM) | |
| 8 | 1 | drop | HEPES | 25 (mM) | |
| 9 | 1 | reservoir | tert-butanol | 20 (%(v/v)) |
