Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 172.022, 172.022, 276.569 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.2543 |
| Rwork | 0.254 |
| R-free | 0.30440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DOO |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.649 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.120 | |
| Total number of observations | 706383 * | |
| Number of reflections | 59863 | |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | A:B=2:1 ratio, Bochtler, M., (2000) Nature, 403, 800. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HslU | 20 (mg/ml) | solutionA |
| 10 | 1 | drop | 1 (mM) | solutionB | |
| 11 | 1 | reservoir | MES | 100 (mM) | |
| 12 | 1 | reservoir | sodium acetate | 2.0 (M) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | solutionA |
| 3 | 1 | drop | EDTA | 1 (mM) | solutionA |
| 4 | 1 | drop | 1 (mM) | solutionA | |
| 5 | 1 | drop | AMP-PNP | 1 (mM) | solutionA |
| 6 | 1 | drop | histidine-tagged HslV | 16 (mg/ml) | solutionB |
| 7 | 1 | drop | 300 (mM) | solutionB | |
| 8 | 1 | drop | Tris-HCl | 20 (mM) | solutionB |
| 9 | 1 | drop | EDTA | 1 (mM) | solutionB |
