1E8U

Structure of the multifunctional paramyxovirus hemagglutinin-neuraminidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	72.560, 78.490, 199.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.232
Rwork	0.232
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.380
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Rmerge	0.071	0.258 *
Total number of observations	282836 *
Number of reflections	75784
Completeness [%]	97.0	98 *
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.6		pH 4.60